In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway. Chapters focus on assays developed to detect its activation not only in vitro but also in vivo, optimized multiplex analysis, medium- to high-throughput screens, and the cellular process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Apoptosis and Cancer: Methods and Protocols, Second Edition aids scientists as a stand-alone resource for the execution and analysis of the described protocols and as a reference for the study and detection of apoptosis within and outside the area of cancer research.

Table of contents (15 chapter)
Caspase-3 Activation Is a Critical Determinant of Genotoxic Stress-Induced Apoptosis
Choudhary, Gaurav S. (et al.)
Pages 1-9
PreviewBuy Chapter 48,34
Flow Cytometry Enumeration of Apoptotic Cancer Cells by Apoptotic Rate
Diaz, David (et al.)
Pages 11-20
Multiplexed Viability, Cytotoxicity, and Caspase Activity Assays
Niles, Andrew L. (et al.)
Pages 21-33
A Multiplexed Method for Kinetic Measurements of Apoptosis and Proliferation Using Live-Content Imaging
Artymovich, Katherine (et al.)
Pages 35-42
Detection of End-Stage Apoptosis by ApopTag
Mohan, Chandra (et al.)
Pages 43-56
Detection and Quantification of Apoptosis in Primary Cells Using Taqman
Pfister, Christina (et al.)
Pages 57-73
Detection of p53 Protein Aggregation in Cancer Cell Lines and Tumor Samples
Yang-Hartwich, Yang (et al.)
Pages 75-86
Detection of p53 Protein Transcriptional Activity by Chromatin Immunoprecipitation
Yang-Hartwich, Yang (et al.)
Pages 87-93
Homogeneous, Bioluminescent Proteasome Assays
O'Brien, Martha A. (et al.)
Pages 95-114
Laser Capture Microdissection for Gene Expression Analysis
Bidarimath, Mallikarjun (et al.)
Pages 115-137
Using the Peggy Simple Western System for Fine Needle Aspirate Analysis
Gentalen, Erik T. (et al.)
Pages 139-155
Analysis of Autophagosome Formation Using Lentiviral Biosensors for Live Fluorescent Cellular Imaging
Long, Kevin (et al.)
Pages 157-169
Optical Imaging of Ovarian Cancer Using HER-2 Affibody Conjugated Nanoparticles
Satpathy, Minati (et al.)
Pages 171-185
Measuring Cardiac Autophagic Flux In Vitro and In Vivo
Gurney, Michael A. (et al.)
Pages 187-197
PET Imaging for Tyrosine Kinase Inhibitor (TKI) Biodistribution in Mice
Fushiki, Hiroshi (et al.)