Table of Contents
Foreword by Robert M. Clegg
1. Proteins and the Flow of Information in Cellular Function, Robert H. Kretsinger2. Basics of Fluorescence and FRET, Zygmunt Gryczynski, Ignacy Gryczynski and Joseph R. Lakowicz3. An Introduction to Filters and Mirrors for FRET, C. Michael Stanley4. FRET Imaging in the Wide-Field Microscope, Fred Schaufele, Ignacio Demarco, and Richard N. Day5. Confocal FRET Microscopy: Study of Clustered Distribution of Receptor-Ligand Complexes in Endocytic Membranes, Horst Wallrabe and Margarida Barroso6. Multiphoton FRET Microscopy for Protein Localization in Tissue, James D. Mills, James R. Stone, David O. Okonkwo, Ammasi Periasamy and Gregory A. Helm7. FRET Data Analysis: The Algorithm, Ye Chen, Masilamani Elangovan, and Ammasi Periasamy8. Photobleaching FRET Microscopy, Anne Kenworthy9. Single-Molecule FRET, Sungchul Hohng and Taekjip Ha10. FRET Measurements Using Multispectral Imaging, Raad Nashmi, Scott E. Fraser, Henry A. Lester, and Mary E. Dickinson11. Real-Time Fluorescence Lifetime Imaging and FRET Using Fast-Gated Image Intensifiers, Glen Redford and Robert M. Clegg12. Streak Fluorescence Lifetime Imaging Microscopy: A Novel Technology for Quantitative FRET Imaging, V. Krishnan Ramanujan, Jian-Hua Zhang, Victoria. E. Centonze, and Brian Herman13. Time-Correlated Single Photon Counting FLIM-FRET Microscopy for Protein Localization, Ye Chen and Ammasi Periasamy14. Bioluminescence Resonance Energy Transfer: Techniques and Potential, Mohammed Soutto, Yao Xu, and Carl H. Johnson15. Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy, Keith M. Berland16. Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy, Eleonora Keating, Claire M. Brown, and Nils O. Petersen