DESCRIPTION:
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

CONTENTS:
1. Quantitative Functional Morphology by Imaging Flow Cytometry
2. Principles of Amnis Imaging Flow Cytometry
3. Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch
4. Applications of Imaging Flow Cytometry for Microalgae
5. The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry
7. Quantitation of Chromosome Damage by Imaging Flow Cytometry
8. Fluorescent In Situ Hybridization in Suspension by Imaging Flow Cytometry
9. Analysis of Nucleocytoplasmic Protein Shuttling by Imaging Flow Cytometry
10. Using Image-Based Flow Cytometry with a FISH-Based FlowRNA Assay to Simultaneously Detect Intracellular TNF-a Protein and mRNA in Monocytes Following LPS Stimulation
11. Multiparametric Characterization of Human T-Cell Immune Synapses by InFlow Microscopy
12. Studying T Cells
13. Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry
14. Using Image-Based Flow Cytometry to Assess Monocyte Oxidized LDL Phagocytosis Capacity
15. Screening for Drugs Against
16. Accurate Assessment of Cell Death by Imaging Flow Cytometry
17. Temporal Heterogeneity in Apoptosis Determined by Imaging Flow Cytometry
18. FlowCam: Quantification and Classification of Phytoplankton by Imaging Flow Cytometry
19. Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry
20. Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes
21. Sickle Cell Imaging Flow Cytometry Assay (SIFCA)