With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.

-Flow Cytometry: An Introduction
Givan, Alice L.
Seiten 1-29
-Breaking the Dimensionality Barrier
Bagwell, C. Bruce
Seiten 31-51
-Quantitative Fluorescence Measurements with Multicolor Flow Cytometry
Wang, Lili (et al.)
Seiten 53-65
-Quantum Dots for Quantitative Flow Cytometry
Buranda, Tione (et al.)
Seiten 67-84
-Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry
Heyde, Henri C. (et al.)
Seiten 85-95
-Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization
Bruckner, Shane (et al.)
Seiten 97-118
-Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry
Tario, Joseph D. (et al.)
Seiten 119-164
-Multiparameter Intracellular Cytokine Staining
Lovelace, Patricia (et al.)
Seiten 165-178
-Phospho Flow Cytometry Methods for the Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples
Krutzik, Peter O. (et al.)
Seiten 179-202
-Multiparametric Analysis of Apoptosis by Flow Cytometry
Telford, William G. (et al.)
Seiten 203-227
-Multiparameter Cell Cycle Analysis
Jacobberger, James W. (et al.)
Seiten 229-249
-Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue
Zimmerlin, Ludovic (et al.)
Seiten 251-273
-Flow Cytometry-Based Identification of Immature Myeloerythroid Development
Pronk, Cornelis J. H. (et al.)
Seiten 275-293
-Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia
Calvo, Katherine R. (et al.)
Seiten 295-316
-Flow Cytometry Assays in Primary Immunodeficiency Diseases
O'Gorman, Maurice R. G. (et al.)
Seiten 317-335
-Flow Cytometric Analysis of Microparticles
Heyde, Henri C. (et al.)
Seiten 337-354
-Noncytotoxic DsRed Derivatives for Whole-Cell Labeling
Strack, Rita L. (et al.)
Seiten 355-370
-Flow Cytometric FRET Analysis of Protein Interaction
Vereb, György (et al.)
Seiten 371-392
-Fluorescent Protein-Assisted Purification for Gene Expression Profiling
Zaidi, M. Raza (et al.)
Seiten 393-405
-Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type
Galbraith, David W. (et al.)
Seiten 407-429
-Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics
Riz, Irene (et al.)
Seiten 431-447
-Standard Practice for Cell Sorting in a BSL-3 Facility
Perfetto, Stephen P. (et al.)
Seiten 449-469
-The Cytometric Future: It Ain't Necessarily Flow!
Shapiro, Howard M.
Seiten 471-482
-Erratum
Hawley, Teresa S. (et al.)