Metabolomics enables new scientific discoveries in the life sciences that make it of increasing interest in biology. Focusing on analysing different small molecules produced or modified by living cells in a high throughput fashion, metabolomics plays an essential role in functional genomics and systems biology studies. Starting with a general introduction to the field, this research-level book addresses the unique challenges associated with microbial metabolomics, such as low biomass concentration and distinction between intra- and extracellular compounds, covering the latest developments in microbial metabolomics before discussing data generation and analysis and applications of quantitative metabolomics.

1. Introduction to metabolomics

Part I - Data generation

2. Quenching techniques for microbial metabolomics
3. Extracting intracellular metabolites from microbial cells
4. Extracellular metabolomics
5. Analytical techniques from the perspective of microbial metabolomics
6. Quantitative microbial metabolomics
7. Metabolomics of microbial communities

Part II - Data processing

8. The statistical planning of metabolomics experiments
9. Data (pre-) processing: When a peak is a metabolite?
10. Statistical exploration and primary analyses of microbial metabolite data
11. Combining metabolomics data with other omics data and extensions to metabolic pathway analysis of microorganisms
12. Modelling metabolic networks of microorganisms and its use in metabolomic data analysis