Part I Isolation of Human Multipotential Stromal Cells
from Bone Marrow and Adipose Tissue
Chapter 1 Isolation and Culture of Bone Marrow-Derived
Human Multipotent Stromal Cells (hMSCs) . . . . . . . . . . . . . 3
Margaret Wolfe, Radhika Pochampally, William Swaney,
and Roxanne L. Reger
Chapter 2 Mesenchymal Stem Cells from Adult Bone Marrow . . . . . . 27
Mark F. Pittenger
Chapter 3 A Method to Isolate and Purify Human Bone
Marrow Stromal Stem Cells . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Stan Gronthos and Andrew C. W. Zannettino
Chapter 4 Adipose-Derived Stem Cells . . . . . . . . . . . . . . . . . . . . . . . . . . 59
John K. Fraser, Min Zhu, Isabella Wulur, and Zeni Alfonso
Chapter 5 Isolation of Human Adipose-derived Stem Cells
from Biopsies and Liposuction Specimens . . . . . . . . . . . . . . . 69
Severine G. Dubois, Elizabeth Z. Floyd, Sanjin Zvonic,
Gail Kilroy, Xiying Wu, Stacy Carling, Yuan Di C. Halvorsen,
Eric Ravussin, and Jeffrey M. Gimble
Part II Manipulation of Human Multipotential Stromal Cells
Chapter 6 Colony Forming Unit Assays for MSCs . . . . . . . . . . . . . . . . 83
Radhika Pochampally
ix
Chapter 7 Differentiation and Characterization
of Human MSCs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe
Chapter 8 Freezing Harvested hMSCs and Recovery of hMSCs
from Frozen Vials for Subsequent Expansion,
Analysis, and Experimentation . . . . . . . . . . . . . . . . . . . . . . . 109
Roxanne L. Reger and Margaret R. Wolfe
Chapter 9 Gene Expression Analysis at the Single Cell Level
Using the Human Bone Marrow Stromal Cell as a Model:
Sample Preparation Methods . . . . . . . . . . . . . . . . . . . . . . . . 117
Beerelli Seshi
Chapter 10 Assays of MSCs with Microarrays . . . . . . . . . . . . . . . . . . . . 133
Joni Ylöstalo, Radhika Pochampally,
and Darwin J. Prockop
Chapter 11 Gene Delivery to Mesenchymal Stem Cells . . . . . . . . . . . . . 153
Reza Izadpanah and Bruce A. Bunnell
Part III Isolation and Characterization of Murine Multipotential
Stromal Cells from Bone Marrow
Chapter 12 Isolation of Mesenchymal Stem Cells from Murine
Bone Marrow by Immunodepletion . . . . . . . . . . . . . . . . . . . 171
Donald G. Phinney
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 187