Hematopoietic stem cell protocols present a small population of adult bone marrow cells that have the capacity to generate the entire blood
and immune system. This text describes the inherent value and limitation of each experimentalapproach.

INDICE: Non-invasive measurement of hematopoietic stem cell cycling and turnover by in vivo bromodeoxyuridine incorporation; isolation and characterization of primitive hematopoietic cells based on their position in cell cycle; isolation and analysis of hematopoietic stem cells from mouse embryos; the purification of mouse hematopoietic stem cells at sequential stages of maturation; method for purification of human hematopoietic stem cells by flow
cytometry; flow cytometry and immunoselection of human stem cells; hematopoietic colony-forming cells; the cobblestone area forming assay CFU-S: an assay for pluripotent myelopoietic stem cells; intrathymic injection for analysis of cell progenitor activity; long-term culture-initiating cell assays for human and murine cells; quantitation of murine and human hematopoietic cells by
limiting-dilution analysis in competitively repopulated hosts; ex vivo expansion of human and murine hematopoietic stem cells; genetic
modification of murine hematopoietic stem cells by retroviruses; retroviral transduction of facs-purified hematopoietic stem cells;
retroviral-mediated transduction and clonal integration analysis of human hematopoietic stem and progenitor cells; production of lentiviral vector supernatants and transduction of cellular targets; reverse transcriptase-pcr analysis of gene expression in hsc; identification of differentially expressed genes in sorted cell
populations by two-dimensional gene expression fingerprinting; Construction of DNA libraries from purified populations of hematopoietic stem cells; hematopoietic development of ES cells in culture.